Comparison of some molecular-genetic techniques for identification of Leishmania circulating in natural foci of zoonotic cutaneous leishmaniasis in the central Asia region.

نویسندگان

  • M V Strelkova
  • R S Pacheco
  • S A Bulat
  • T A Rakitskaya
چکیده

Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization techniques are discussed in terms of their applicability, discrimination power and feasibility for answering questions related to molecular epidemiological research and for detecting mixed Leishmania infections.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Detection and identification of Leishmania isolates from patients with Cutaneous Leishmaniasis (CL) in Isfahan (central region of Iran) by PCR method

Leishmaniasis is caused by parasitic protozoa of the genus Leishmania. Cutaneous leishmaniasis (CL) is a complex disease with wide spectrum of clinical manifestations. In order to identify leishmania species causing CL in Isfahan by a definite molecular technique (PCR method), this study was undertaken over 2010- 2011. 124 Patients with suspicious lesion of Leishmaniasis and positive direct sme...

متن کامل

Different Morphologies of Leishmania major Amastigotes with No Molecular Diversity in a Neglected Endemic Area of Zoonotic Cutaneous Leishmaniasis in Iran

Background: Molecular diversity of Leishmania major and its morphological changes have become a controversial issue among researchers. Some aspects of polymorphic shapes of amastigotes in clinical manifestations along with molecular variation were evaluated among suspected patients of some exceptional zoonotic cutaneous leishmaniasis locations in Northern Khuzestan, Southwestern Iran. Methods: ...

متن کامل

Identification of Leishmania species isolated from paitients with cutaneous leishmaniasis in Ghohab Mohammad Abad Isfahan using PCR method

Background and aim: Cutaneous leishmaniasis (CL) is a health problem of many countries in tropical and subtropical regions including Iran. Isfahan province is one of the foci of CL with the highest prevalence in Iran. This study was done to identify the species of Leishmania isolated from the patients in Ghohab Mohammad Abad, located in southwest of Isfahan which is a new foci of CL. Identifica...

متن کامل

Identification of Leishmania species using monoclonal antibodies in Isfahan

Background: Isfahan is one of the major foci of cutaneous leishmaniasis in Iran. According to the epidemiological studies, both urban and rural forms have been reported in Isfahan. Accurate identification and characterization of Leishmania is essential for the control of the disease. Several different molecular techniques for Leishmania identification are developed including use of monoclonal a...

متن کامل

Isolation and Molecular Identification of Leishmania spp. in Patients With Cutaneous Leishmaniasis in Golestan Province, Iran

Background and aims: Cutaneous leishmaniasis is a major health issue in many parts of Iran.Diagnosis of cutaneous leishmaniasis in a non-endemic area is not as simple as that in endemic foci.The management and treatment of this disease are global dilemmas. The purpose of this study was toidentify Leishmania species isolated from human cutaneous leishmaniasis lesions in patient...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Memorias do Instituto Oswaldo Cruz

دوره 92 1  شماره 

صفحات  -

تاریخ انتشار 1997